BLV PVL Calculator

This web-based tool is designed to support standard curve calibration for qPCR instruments using the STRATA-G™ Bovine Leukemia Virus PCR Precision DNA Standards and derive target copy number from Cycle threshold (Ct) values.  

Copy number estimates for Bovine Leukemia Virus (BLV) and β-actin (βA) are used to calculate Proviral load (PVL). 

Calibration Calculator

Click BLV Calibration, enter Ct values from the standard curve replicates and click calculate. Record the “A” and “B” values.  

PVL Calculator  

To calculate copy numbers and PVLs for your samples, paste a tabular dataset with three columns: Sample Name, BLV Ct, and βA Ct, into the designated sample input section at the bottom of the page.

Note: This tool is optimized for desktop or laptop use and is not supported on mobile devices.

This tool is intended for research use only and is not intended for clinical or diagnostic applications. Results generated by the BLV PVL Calculator are provided for informational purposes and should be verified independently prior to formal reporting of results to third parties. 

Calibrations

Y = × X + R2 =
R1 R2 R3 Average Standard Deviation
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1 Copy

Y = × X + R2 =
R1 R2 R3 Average Standard Deviation
1,000,000 Copies
100,000 Copies
10,000 Copies
1,000 Copies
100 Copies
10 Copies
1 Copy